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rhesus macaque epithelial cells llc mk2  (ATCC)


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    ATCC rhesus macaque epithelial cells llc mk2
    Rhesus Macaque Epithelial Cells Llc Mk2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/llc+mk2+cells/LLC-MK2+Original/pm42274213-350-71-92
    Average 99 stars, based on 918 article reviews
    rhesus macaque epithelial cells llc mk2 - by Bioz Stars, 2026-09
    99/100 stars

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    other:

    Article Title: Molecular basis for protection and cross-protection by human antibodies targeting the parainfluenza virus hemagglutinin-neuraminidase protein
    Article Snippet: Freestyle293-F cells (ThermoFisher, Cat: R79007) were cultured in Freestyle 293 media (ThermoFisher, Cat: 12338026).

    Suspension:

    Article Title: A novel antiviral strategy targeting human metapneumovirus through pH modulation in human airway epithelial cells
    Article Snippet: A green fluorescent protein-expressing recombinant human metapneumovirus (rhMPV-GFP; ViraTree, MPV-GFP1, #M121, CAN97-83 [A2]) was used at virus passages 4-5. .. Viral stocks were propagated in LLC-MK2 cells (ATCC CCL-7) and stored in suspension medium consisting of Opti-MEM, sucrose, and L-glutamic acid ( ). ..

    Cell Culture:

    Article Title: Variable and conserved features of copy-back viral genome populations generated de novo during Sendai virus infection
    Article Snippet: .. BSR-T7/5 cells (kindly provided by Dr. Conzelmann) , LLC-MK2 cells (ATCC, #CCL-7), and A549 cells (ATCC, #CCL-185) were cultured in tissue culture medium (Dulbecco’s modified Eagle’s medium) (Invitrogen,#11965092) supplemented with 10% fetal bovine serum (Sigma, #F0926), gentamicin 50 ng/mL (ThermoFisher, #15750060), L-glutamine 2 mM (Invitrogen, #G7513), and sodium pyruvate 1 mM (Invitrogen, #25-000-C1) at 5% CO 2 37°C. .. Cells were treated with mycoplasma removal agent (MP Biomedical, #3050044) and tested monthly for mycoplasma contamination using the MycoAlert Plus mycoplasma testing kit (Lonza, #LT07-318).

    Article Title: Development of a potent monoclonal antibody for treatment of human metapneumovirus infections.
    Article Snippet: 293F cells (Thermo Fisher, cat#R79007) were cultured in Freestyle 293 media (Thermo Fisher, cat#12338026). .. Vero cells (ATCC CCL-81) and LLC-MK2 cells (ATCC CCL-7.1) were cultured in DMEM (Gibco, cat#12430054) supplemented with 10% fetal bovine serum and 100 U/mL penicillin plus 100 μg/mL streptomycin (Gibco, cat#15140122). ..

    Article Title: Development of a potent monoclonal antibody for treatment of human metapneumovirus infections
    Article Snippet: 293F cells (Thermo Fisher, cat# R79007 ) were cultured in Freestyle 293 media (Thermo Fisher, cat#12338026). .. Vero cells (ATCC CCL-81) and LLC-MK2 cells (ATCC CCL-7.1) were cultured in DMEM (Gibco, cat#12430054) supplemented with 10% fetal bovine serum and 100 U/mL penicillin plus 100 μg/mL streptomycin (Gibco, cat#15140122). ..

    Article Title: Universal broad-spectrum mucosal vaccine design for human coronaviruses inspired by artificial antibodies.
    Article Snippet: VeroE6 (American Type Culture Collection [ATCC], Manassas, VA, USA) cells were cultured in Dulbecco modified eagle medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) at 37 °C and 5% CO2 for passage, titration, and neutralization of SARS-CoV-2. .. LLC-MK2 cells (ATCC) were cultured in DMEM supplemented with 10% FBS at 35 °C and 5% CO2 for passage, titration, and neutralization of HCoV NL63, according to standard protocols. ..

    Modification:

    Article Title: Variable and conserved features of copy-back viral genome populations generated de novo during Sendai virus infection
    Article Snippet: .. BSR-T7/5 cells (kindly provided by Dr. Conzelmann) , LLC-MK2 cells (ATCC, #CCL-7), and A549 cells (ATCC, #CCL-185) were cultured in tissue culture medium (Dulbecco’s modified Eagle’s medium) (Invitrogen,#11965092) supplemented with 10% fetal bovine serum (Sigma, #F0926), gentamicin 50 ng/mL (ThermoFisher, #15750060), L-glutamine 2 mM (Invitrogen, #G7513), and sodium pyruvate 1 mM (Invitrogen, #25-000-C1) at 5% CO 2 37°C. .. Cells were treated with mycoplasma removal agent (MP Biomedical, #3050044) and tested monthly for mycoplasma contamination using the MycoAlert Plus mycoplasma testing kit (Lonza, #LT07-318).

    In Vitro:

    Article Title: Antiviral heterocyclic compounds
    Article Snippet: TABLE 13 Summary of Activities for HMPV HMPV HMPV Compound EC50 Compound EC50 3 B 4 A 6 A 7 A 8 A 9 A 10 A 11 B 12 A 13 A 14 A 16 A 17 A 19 A 20 A 22 A 23 A 24 A 25 A 26 A 27 A 28 A 29 A 30 A 32 A 34 B 40 A 41 A 42 A 43 A 44 A 45 A 47 A 48 A 49 B 50 B 51 A 52 A 53 A 54 B 55 A 57 B 58 A 59 A 60 A 62 B 63 B 64 B 65 B 68 A 70 A 72 A 73 A 74 A 75 B 77 A 78 B 80 A 81 B 82 A 83 A 84 A 85 A 86 A 91 B 92 A 94 B 95 B 96 A 97 B 100 B 102 A 103 A 105 B 108 B 112 B 113 A 114 A 120 B 121 A 124 A 128 A 129 B 132 B 134 B 135 B 136 B 137 B 138 B 139 B 141 B 142 B 143 A 147 A 148 A 149 B 152 B 153 B 154 B 155 B 156 B 157 B 158 B 160 B 161 B 165 B 166 B 167 B 168 A 169 B 170 A 174 B 177 A 178 B 179 B 182 A 183 B 184 B 199 B 200 B 210 A 217 A 218 A 219 A 220 B 221 B 222 B 223 B 224 B 226 B 234 A 235 A 263 A 265 A 267 A 268 B 270 A 280 A 300 A 301 A 305 A 306 A 307 A 308 A 309 B 314 A 315 A 321 A 322 B 323 B 324 A 327 B 329 A 441 B Method-B: In vitro HMPV antiviral activity was evaluated using the clinical isolate TN/1501/A1 and LLC-MK2 cells (ATCC #CCL-7), an immortalized kidney epithelial cell line from Macaca mulatta. .. TABLE 13 Summary of Activities for HMPV HMPV HMPV Compound EC50 Compound EC50 3 B 4 A 6 A 7 A 8 A 9 A 10 A 11 B 12 A 13 A 14 A 16 A 17 A 19 A 20 A 22 A 23 A 24 A 25 A 26 A 27 A 28 A 29 A 30 A 32 A 34 B 40 A 41 A 42 A 43 A 44 A 45 A 47 A 48 A 49 B 50 B 51 A 52 A 53 A 54 B 55 A 57 B 58 A 59 A 60 A 62 B 63 B 64 B 65 B 68 A 70 A 72 A 73 A 74 A 75 B 77 A 78 B 80 A 81 B 82 A 83 A 84 A 85 A 86 A 91 B 92 A 94 B 95 B 96 A 97 B 100 B 102 A 103 A 105 B 108 B 112 B 113 A 114 A 120 B 121 A 124 A 128 A 129 B 132 B 134 B 135 B 136 B 137 B 138 B 139 B 141 B 142 B 143 A 147 A 148 A 149 B 152 B 153 B 154 B 155 B 156 B 157 B 158 B 160 B 161 B 165 B 166 B 167 B 168 A 169 B 170 A 174 B 177 A 178 B 179 B 182 A 183 B 184 B 199 B 200 B 210 A 217 A 218 A 219 A 220 B 221 B 222 B 223 B 224 B 226 B 234 A 235 A 263 A 265 A 267 A 268 B 270 A 280 A 300 A 301 A 305 A 306 A 307 A 308 A 309 B 314 A 315 A 321 A 322 B 323 B 324 A 327 B 329 A 441 B Method-B: In vitro HMPV antiviral activity was evaluated using the clinical isolate TN/1501/A1 and LLC-MK2 cells (ATCC #CCL-7), an immortalized kidney epithelial cell line from Macaca mulatta. .. The compounds were diluted and transferred onto 384-well assay plates using the Echo-650 automated liquid handling system (Beckman Coulter, Indiana).

    Activity Assay:

    Article Title: Antiviral heterocyclic compounds
    Article Snippet: TABLE 13 Summary of Activities for HMPV HMPV HMPV Compound EC50 Compound EC50 3 B 4 A 6 A 7 A 8 A 9 A 10 A 11 B 12 A 13 A 14 A 16 A 17 A 19 A 20 A 22 A 23 A 24 A 25 A 26 A 27 A 28 A 29 A 30 A 32 A 34 B 40 A 41 A 42 A 43 A 44 A 45 A 47 A 48 A 49 B 50 B 51 A 52 A 53 A 54 B 55 A 57 B 58 A 59 A 60 A 62 B 63 B 64 B 65 B 68 A 70 A 72 A 73 A 74 A 75 B 77 A 78 B 80 A 81 B 82 A 83 A 84 A 85 A 86 A 91 B 92 A 94 B 95 B 96 A 97 B 100 B 102 A 103 A 105 B 108 B 112 B 113 A 114 A 120 B 121 A 124 A 128 A 129 B 132 B 134 B 135 B 136 B 137 B 138 B 139 B 141 B 142 B 143 A 147 A 148 A 149 B 152 B 153 B 154 B 155 B 156 B 157 B 158 B 160 B 161 B 165 B 166 B 167 B 168 A 169 B 170 A 174 B 177 A 178 B 179 B 182 A 183 B 184 B 199 B 200 B 210 A 217 A 218 A 219 A 220 B 221 B 222 B 223 B 224 B 226 B 234 A 235 A 263 A 265 A 267 A 268 B 270 A 280 A 300 A 301 A 305 A 306 A 307 A 308 A 309 B 314 A 315 A 321 A 322 B 323 B 324 A 327 B 329 A 441 B Method-B: In vitro HMPV antiviral activity was evaluated using the clinical isolate TN/1501/A1 and LLC-MK2 cells (ATCC #CCL-7), an immortalized kidney epithelial cell line from Macaca mulatta. .. TABLE 13 Summary of Activities for HMPV HMPV HMPV Compound EC50 Compound EC50 3 B 4 A 6 A 7 A 8 A 9 A 10 A 11 B 12 A 13 A 14 A 16 A 17 A 19 A 20 A 22 A 23 A 24 A 25 A 26 A 27 A 28 A 29 A 30 A 32 A 34 B 40 A 41 A 42 A 43 A 44 A 45 A 47 A 48 A 49 B 50 B 51 A 52 A 53 A 54 B 55 A 57 B 58 A 59 A 60 A 62 B 63 B 64 B 65 B 68 A 70 A 72 A 73 A 74 A 75 B 77 A 78 B 80 A 81 B 82 A 83 A 84 A 85 A 86 A 91 B 92 A 94 B 95 B 96 A 97 B 100 B 102 A 103 A 105 B 108 B 112 B 113 A 114 A 120 B 121 A 124 A 128 A 129 B 132 B 134 B 135 B 136 B 137 B 138 B 139 B 141 B 142 B 143 A 147 A 148 A 149 B 152 B 153 B 154 B 155 B 156 B 157 B 158 B 160 B 161 B 165 B 166 B 167 B 168 A 169 B 170 A 174 B 177 A 178 B 179 B 182 A 183 B 184 B 199 B 200 B 210 A 217 A 218 A 219 A 220 B 221 B 222 B 223 B 224 B 226 B 234 A 235 A 263 A 265 A 267 A 268 B 270 A 280 A 300 A 301 A 305 A 306 A 307 A 308 A 309 B 314 A 315 A 321 A 322 B 323 B 324 A 327 B 329 A 441 B Method-B: In vitro HMPV antiviral activity was evaluated using the clinical isolate TN/1501/A1 and LLC-MK2 cells (ATCC #CCL-7), an immortalized kidney epithelial cell line from Macaca mulatta. .. The compounds were diluted and transferred onto 384-well assay plates using the Echo-650 automated liquid handling system (Beckman Coulter, Indiana).

    Titration:

    Article Title: Universal broad-spectrum mucosal vaccine design for human coronaviruses inspired by artificial antibodies.
    Article Snippet: VeroE6 (American Type Culture Collection [ATCC], Manassas, VA, USA) cells were cultured in Dulbecco modified eagle medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) at 37 °C and 5% CO2 for passage, titration, and neutralization of SARS-CoV-2. .. LLC-MK2 cells (ATCC) were cultured in DMEM supplemented with 10% FBS at 35 °C and 5% CO2 for passage, titration, and neutralization of HCoV NL63, according to standard protocols. ..

    Neutralization:

    Article Title: Universal broad-spectrum mucosal vaccine design for human coronaviruses inspired by artificial antibodies.
    Article Snippet: VeroE6 (American Type Culture Collection [ATCC], Manassas, VA, USA) cells were cultured in Dulbecco modified eagle medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) at 37 °C and 5% CO2 for passage, titration, and neutralization of SARS-CoV-2. .. LLC-MK2 cells (ATCC) were cultured in DMEM supplemented with 10% FBS at 35 °C and 5% CO2 for passage, titration, and neutralization of HCoV NL63, according to standard protocols. ..



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    Differential IRGs and ISGs expressed during hMPV infection. A Heatmap of differentially expressed IRGs and ISGs with P values of < 0.05 in the hMPV group versus the control group and the RSV group. B Detailed expression levels of IRGs and ISGs with P values < 0.05 in the three groups. Statistical analysis was performed with an unpaired t -test. P values of 0.05 (∗), 0.01 (∗∗), and 0.001 (∗∗∗) were considered significant; NS: not significant. C Real-time PCR quantification of selected IRGs in infected and control cells at 24, 48, and 72 h postinfection. RSV and hMPV were propagated <t>in</t> <t>LLC-MK2</t> cells at a multiplicity of infection (MOI) of 0.1. N = 3. Statistical analysis was performed with an unpaired t -test. P values < 0.05 (∗), < 0.01 (∗∗), and < 0.0001 (∗∗∗∗) were considered significant. The bars in the figure panels show the means and standard errors of the means (SEMs).
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    Differential IRGs and ISGs expressed during hMPV infection. A Heatmap of differentially expressed IRGs and ISGs with P values of < 0.05 in the hMPV group versus the control group and the RSV group. B Detailed expression levels of IRGs and ISGs with P values < 0.05 in the three groups. Statistical analysis was performed with an unpaired t -test. P values of 0.05 (∗), 0.01 (∗∗), and 0.001 (∗∗∗) were considered significant; NS: not significant. C Real-time PCR quantification of selected IRGs in infected and control cells at 24, 48, and 72 h postinfection. RSV and hMPV were propagated <t>in</t> <t>LLC-MK2</t> cells at a multiplicity of infection (MOI) of 0.1. N = 3. Statistical analysis was performed with an unpaired t -test. P values < 0.05 (∗), < 0.01 (∗∗), and < 0.0001 (∗∗∗∗) were considered significant. The bars in the figure panels show the means and standard errors of the means (SEMs).
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    Differential IRGs and ISGs expressed during hMPV infection. A Heatmap of differentially expressed IRGs and ISGs with P values of < 0.05 in the hMPV group versus the control group and the RSV group. B Detailed expression levels of IRGs and ISGs with P values < 0.05 in the three groups. Statistical analysis was performed with an unpaired t -test. P values of 0.05 (∗), 0.01 (∗∗), and 0.001 (∗∗∗) were considered significant; NS: not significant. C Real-time PCR quantification of selected IRGs in infected and control cells at 24, 48, and 72 h postinfection. RSV and hMPV were propagated <t>in</t> <t>LLC-MK2</t> cells at a multiplicity of infection (MOI) of 0.1. N = 3. Statistical analysis was performed with an unpaired t -test. P values < 0.05 (∗), < 0.01 (∗∗), and < 0.0001 (∗∗∗∗) were considered significant. The bars in the figure panels show the means and standard errors of the means (SEMs).
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    Differential IRGs and ISGs expressed during hMPV infection. A Heatmap of differentially expressed IRGs and ISGs with P values of < 0.05 in the hMPV group versus the control group and the RSV group. B Detailed expression levels of IRGs and ISGs with P values < 0.05 in the three groups. Statistical analysis was performed with an unpaired t -test. P values of 0.05 (∗), 0.01 (∗∗), and 0.001 (∗∗∗) were considered significant; NS: not significant. C Real-time PCR quantification of selected IRGs in infected and control cells at 24, 48, and 72 h postinfection. RSV and hMPV were propagated in LLC-MK2 cells at a multiplicity of infection (MOI) of 0.1. N = 3. Statistical analysis was performed with an unpaired t -test. P values < 0.05 (∗), < 0.01 (∗∗), and < 0.0001 (∗∗∗∗) were considered significant. The bars in the figure panels show the means and standard errors of the means (SEMs).

    Journal: Virologica Sinica

    Article Title: Metatranscriptomics uncovers host immune and microbiome signatures specific to and shared between human metapneumovirus and respiratory syncytial virus infections in children

    doi: 10.1016/j.virs.2026.03.002

    Figure Lengend Snippet: Differential IRGs and ISGs expressed during hMPV infection. A Heatmap of differentially expressed IRGs and ISGs with P values of < 0.05 in the hMPV group versus the control group and the RSV group. B Detailed expression levels of IRGs and ISGs with P values < 0.05 in the three groups. Statistical analysis was performed with an unpaired t -test. P values of 0.05 (∗), 0.01 (∗∗), and 0.001 (∗∗∗) were considered significant; NS: not significant. C Real-time PCR quantification of selected IRGs in infected and control cells at 24, 48, and 72 h postinfection. RSV and hMPV were propagated in LLC-MK2 cells at a multiplicity of infection (MOI) of 0.1. N = 3. Statistical analysis was performed with an unpaired t -test. P values < 0.05 (∗), < 0.01 (∗∗), and < 0.0001 (∗∗∗∗) were considered significant. The bars in the figure panels show the means and standard errors of the means (SEMs).

    Article Snippet: Adult rhesus monkey kidney cells (LLC-MK2) (CCL-7) were obtained from ATCC (Manassas, VA, USA).

    Techniques: Infection, Control, Expressing, Real-time Polymerase Chain Reaction